Prourokinase Mutant That Induces Highly Effective Clot Lysis Without Interfering With Hemostasis
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چکیده
منابع مشابه
Prourokinase mutant that induces highly effective clot lysis without interfering with hemostasis.
Prourokinase (proUK) is a zymogenic plasminogen activator that at pharmacological doses is prone to nonspecific activation to urokinase. This has handicapped therapeutic exploitation of its fibrin-specific physiological properties. To attenuate this susceptibility without compromising specific activation of proUK on a fibrin clot, a Lys300-->His mutation (M5) was developed. M5 had a lower intri...
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Platelet facilitation of clot lysis was studied using the dilute clot lysis assay, a standardized assay for fibrinolysis shown to correlate with the development of postoperative deep vein thrombosis. Clots prepared from dilute platelet poor plasma showed prolonged clot lysis when compared with clots prepared in a similar fashion from dilute platelet rich plasma. Since in the presence of platele...
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Currently, the study of fibrinolysis is beset by problems of methodology (1 ). Disadvantages of many of the present methods for measurement of clot lysisincluding lysis time for the dilute blood clot, thromboelastography, euglobulin clot lysis, fibrin plate method, and caseinolytic assay (1 )-are imprecision of results and time required to perform the test (2). The electromechanical lysis timer...
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The principles relating the lysis times of fibrin clots to their contents of fibrin, plasminogen and plasminogen-activator were investigated. Mathematical considerations suggested that the square of the lysis time should correlate linearly with the fibrin content, and inversely with the activator and the plasminogen contents of the system. Experimental studies, during which these parameters wer...
متن کاملA new device for measurement of fibrin clot lysis: application to the Euglobulin Clot Lysis Time
BACKGROUND Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely computerised semi-automatic 8-channel device for measurement and determination of fibrin clot lysis. The lysis time is evaluated by a mathematical analysis of the lysis curve a...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 2002
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.0000014825.71092.bd